Prototype
Benchtop Static Light Scattering
Optical biosensing, scattering analysis, low-cost instrumentation
Low-cost optical setup to perform static light scattering experiments.
The project proposes the novel design of apparatus designed to perform static light scattering on biological samples. The AS7431 spectral sensor was used to capture the intensity of scattered, wavelength-specific light. The SLS module was evaluated by analysing saliva samples from 7 participants, 5 male and 2 female under resting and active conditions. Using the small-angle scattering intensities acquired across different angles, data fitting was performed using a linearised Guinier approximation to attain Rg and R^2 (coefficient of determination). This was used to assess the fit. The estimated Rg values derived from the device are well within the expected range of salivary mucins, as reported by [Kesimer M, Sheehan JK et al].
Built alongside: Michaela Lastovickova (mpzh0862@leeds.ac.uk).
Highlights
- Designed a low-cost benchtop SLS apparatus for biological samples.
- Captured wavelength-specific scattering with the AS7431 spectral sensor.
- Applied Guinier fits to estimate Rg and assess model fit.
Tools and Methods
- 3D printing
- Optical lenses
- Laser diode
- Photodiodes
- Variable LEDs
- Optical mounts
- Apertures and collimators